Suspension-Vero cell cultures as a platform for viral vaccine production

详细信息    查看全文

• 作者：Cristian Paillet ; Guillermina Forno ; Ricardo Kratje ; Marina Etcheverrigaray
• 关键词：Vero cells ; Vaccines ; Suspension culture
• 刊名：Vaccine
• 出版年：2009
• 出版时间：30 October 2009
• 年：2009
• 卷：27
• 期：46
• 页码：6464-6467
• 全文大小：292 K

文摘

Since Vero cells are currently considered as an acceptable cell substrate to produce a wide range of viruses, we developed a virus production platform using Vero cells adapted to grow in suspension in serum-free media. After adapting anchorage-dependent Vero cells to grow as a free-cell suspension, vesicular stomatitis virus, herpes simplex virus 1 and polio virus 1 production rates were evaluated in batch cultures using spinner flasks and perfused cultures in a bioreactor. The achieved results constitute valuable information for the development of a low-cost high-productivity process using a suspension culture of Vero cells to produce viral vaccines.