The Impact of Chlorhexidine-based Endodontic Treatment on Periapical Cytokine Expression in Teeth

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• 作者：Warley Luciano Fonseca Tavares ; DDS ; PhD^&lowast ; ; Luciana Carla Neves de Brito ; DDS ; PhD^&lowast ; ; Luiz Carlos Feitosa Henriques ; DDS ; PhD^&lowast ; ; Ricardo Reis Oliveira ; DDS ; PhD^&lowast ; ; Kamilla Faria Maciel ; DDS^&lowast ; ; Leda Qué ; rcia Vieira ; PhD^&dagger ; ; Antô ; nio Paulino Ribeiro Sobrinho ; DDS ; PhD^&lowast ; ; ^{sobrinho.bhz@terra.com.br" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Chlorhexidine ; cytokine expression ; root canal infection
• 刊名：Journal of Endodontics
• 出版年：2013
• 出版时间：July 2013
• 年：2013
• 卷：39
• 期：7
• 页码：889-892
• 全文大小：281 K

文摘

Root canal treatment typically involves cleaning and shaping procedures followed by treatment with antibacterial endodontic dressing between appointments and, ultimately, 3-dimensional,hermetic filling. Chlorhexidine (CHX) is effective as an irrigation solution and is used as an endodontic dressing. The aim of this study was to examine the influence of CHX on periapical cytokine expression.

Methods

Expression levels of the cytokines interferon γ, tumor necrosis factor α, interleukin (IL)-1β, IL-17A, IL-10, and the chemokine monocyte chemoattractant protein-1 (CCL2/MCP-1) were assayed by real-time polymerase chain reaction immediately after root canal cleaning and 15 days later.

Results

Messenger RNA expression of IL-1β, interferon γ, IL-10, and CCL2/MCP-1 was increased on day 15 in teeth without endodontic dressing. No statistical change was observed in the messenger RNA expression of cytokines when comparing sampling times for teeth that received endodontic dressing.

Conclusions

The results show that CHX application between appointments prevented the increase of both proinflammatory and immunoregulatory cytokines 15 days after the dental procedure.