Ready-to-drink Matte庐 tea (diet and regular) increased life span and pulmonary health in aged mice
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- 作者：Manuella Lanzetti ; Karla Maria Pereira Pires ; Juliana Cardoso Santos ; Marcelo Lima Ribeiro ; Ricardo Moreira Borges ; Ant么nio Jorge Ribeiro da Silva ; Lu铆s Crist贸v茫o Porto ; Cl谩udia Farias Benjamim ; Samuel Santos Valenca
- 关键词：Aging ; Matte tea ; Oxidative stress ; Inflammation ; Lung ; Mouse
- 刊名：Food Research International
- 出版年：November, 2013
- 年：2013
- 卷：54
- 期：1
- 页码：675-682
- 全文大小：794 K
文摘
Aging coexists with increased oxidant production. Increasing the amount of antioxidants due to Matte tea intake may therefore modulate the aging process in mouse lungs. Our aim was to study the effects of ready-to-drink Matte庐 tea, both diet (DMT) and regular (RMT), in antioxidant profile and inflammatory markers of aged mouse lungs. Twelve-month-old male C57BL/6 mice received either DMT (n = 10) or RMT (n = 10) daily for 6 months. Control mice (n = 10) received mineral water. Mice were then sacrificed and bronchoalveolar lavage (BAL) and lungs were collected to analyze oxidative stress and inflammatory markers. Both the DMT and RMT groups were compared with the Control group. ROS levels were 50% decreased in the DMT group and 50% increased in the RMT group. Nitrite levels were 50% decreased in both DMT and RMT groups. Superoxide dismutase (SOD) activity was similar among groups. However, both catalase (CAT) and glutathione peroxidase (GPx) activities were 31% and 90% increased in the DMT and 25% and 90% in the RMT groups, respectively. Reduced glutathione/oxidized glutathione ratio was 86% increased in the DMT group. Both DMT and RMT groups showed a significant decrease of antioxidant enzyme expressions (SOD 鈥?40%; CAT 鈥?45%; GPx 鈥?50% and 40%) and an increase of SIRT1 (70% and 59%) and Nrf2 (74% and 33%). Both DMT and RMT reduced TNF-伪 (50%), IL-6 (35%) and iNOS (30% and 60%, respectively) gene expressions. The present data suggest that Matte庐 tea may act as an important resource aiming the reduction of lung aging-related oxidative stress by modulating antioxidant defenses.