Comparison of vinblastine-induced micronuclei chromosome malsegregation in binucleated lymphocytes from young and elderly individuals

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• 作者：Leopardi ; Paola ; Marcon ; Francesca ; Dobrowolny ; Gabriella ; Crebelli ; Riccardo ; Zijno ; Andrea
• 关键词：Aneuploidy ; Chromosome X ; Cytokinesis block method
• 刊名：Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis
• 出版年：1997
• 出版时间：September, 1997
• 年：1997
• 卷：379
• 期：1, Supplement 1
• 页码：S135
• 全文大小：110 K

文摘

Aging is an important factor affecting spontaneous levels of aneuploidy in humans. The basic mechanism for this phenomenon and its influence on the susceptibility to chemically-induced aneuploidy have not yet been elucidated. In order to investigate the effect of aging on the susceptibility to chemically-induced malsegregation, three experiments were carried out comparing the effects produced by vinblastine in cytokinesis-blocked lymphocytes of paired young (under 30) and elderly (over 50) male and female donors. Micronuclei and malsegregation of chromosomes X and 8, detected by FISH with centromere specific DNA probes, were analyzed after 60 hours of culture.