IgD cross-linking induces gene expression profiling changes and enhances apoptosis in chronic lymphocytic leukemia cells

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• 作者：Simona Tavolaro¹ ; Nadia Peragine¹ ; Sabina Chiaretti ; Maria Rosaria Ricciardi ; Sara Raponi ; Monica Messina ; Simona Santangelo ; Marilisa Marinelli ; Valeria Di Maio ; Francesca Romana Mauro ; Ilaria Del Giudice ; Robin Foà ; ² ; ^{rfoa@bce.uniroma1.it} ; Anna Guarini²
• 关键词：CLL ; B-cell receptor ; Antigenic stimulation ; Microarray ; Cell cycle ; Apoptosis
• 刊名：Leukemia Research
• 出版年：2013
• 出版时间：April, 2013
• 年：2013
• 卷：37
• 期：4
• 页码：455-462
• 全文大小：926 K

文摘

Gene profile and functional changes upon IgD cross-linking were evaluated in chronic lymphocytic leukemia (CLL). Microarrays highlighted responsiveness to IgD in all cases, independently of clinico-biological characteristics. Stimulated samples exhibited the down-regulation of transcripts of B-cell receptor signaling and cell-adhesion at 24 h and the up-modulation of differentiation and apoptosis genes at 48 h. A significant increase in apoptosis upon ligation was also documented. Furthermore, comparison between IgD and IgM stimulation displayed a differential transcriptional/functional response.

In conclusion, CLL respond to IgD displaying expression changes and cell-death enhancement, indicating the apoptosis induction via-IgD as an alternative approach for CLL management.