Recombinant capripoxviruses expressing proteins of bluetongue virus: Evaluation of immune responses and protection in small ruminants
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- 作者：Auré ; lie Perrin ; Emmanuel Albina ; Emmanuel Bré ; ard ; Corinne Sailleau ; Sylvie Promé ; Colette Grillet ; Olivier Kwiatek ; Pierre Russo ; Richard Thié ; ry ; Stephan Zientara ; Catherine C
- 关键词：Bluetongue ; Capripoxvirus ; Vaccine
- 刊名：Vaccine
- 出版年：2007
- 出版时间：17 September 2007
- 年：2007
- 卷：25
- 期：37-38
- 页码：6774-6783
- 全文大小：381 K
文摘
The development of recombinant capripoxviruses for protective immunization of ruminants against bluetongue virus (BTV) infection is described. Sheep (n = 11) and goats (n = 4) were immunized with BTV recombinant capripoxviruses (BTV-Cpox) individually expressing four different genes encoding two capsid proteins (VP2 and VP7) and two non-structural proteins (NS1, NS3) of BTV serotype 2 (BTV-2). Seroconversion was observed against NS3, VP7 and VP2 in both species and a lymphoproliferation specific to BTV antigens was also demonstrated in goats. Finally, partial protection of sheep challenged 3 weeks after BTV-Cpox administration with a virulent strain of BTV-2, was observed.