Induction of mitochondrial nitrative damage and cardiac dysfunction by chronic provision of dietary ω-6 polyunsaturated fatty acids
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- 作者：Sanjoy Ghosh ; Girish Kewalramani ; Gloria Yuen ; Thomas Pulinilkunnil ; Ding An ; Sheila M. Innis ; Michael F. Allard ; Richard B. Wambolt ; Dake Qi ; Ashraf Abrahani and Brian Rodrigues
- 关键词：ω ; -6 PUFA ; Mitochondria ; DNA ; High fat ; Peroxynitrite ; OGG-1
- 刊名：Free Radical Biology and Medicine
- 出版年：2006
- 出版时间：1 November 2006
- 年：2006
- 卷：41
- 期：9
- 页码：1413-1424
- 全文大小：558 K
文摘
Increased awareness of obesity has led to a dietary shift toward “heart-friendly” vegetable oils containing ω-6 polyunsaturated fatty acid (ω-6 PUFA). In addition to its beneficial effects, ω-6 PUFA also exhibits proinflammatory and prooxidative properties. We hypothesized that chronic dietary ω-6 PUFA can induce free radical generation, predisposing the cardiac mitochondria to oxidative damage. Male Wistar rats were fed a diet supplemented with 20 % w/w sunflower oil, rich in ω-6 PUFA (HP) or normal laboratory chow (LP) for 4 weeks. HP feeding augmented phospholipase A2 activity and breakdown of cardiolipin, a mitochondrial phospholipid. HP hearts also demonstrated elevated inducible nitric oxide synthase expression, loss of Mn superoxide dismutase, and increased mitochondrial nitrotyrosine levels. In these hearts, oxidative damage to mitochondrial DNA (mDNA) was demonstrated by 8-hydroxyguanosine immunopositivity, overexpression of DNA repair enzymes, and a decrease in the mRNA expression of specific respiratory subunits encoded by the mDNA. Functionally, at higher workloads, HP hearts also demonstrated a greater decline in cardiac work than LP, suggesting a compromised mitochondrial reserve. Our study, for the first time, demonstrates that consumption of a high fat diet rich in ω-6 PUFA for only 4 weeks instigates mitochondrial nitrosative damage and causes cardiac dysfunction at high afterloads.