Toxicity of ricin A chain is reduced in mammalian cells by inhibiting its interaction with the ribosome
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- 作者：Amanda E. Jetzta ; Xiao-Ping Lib ; Nilgun E. Tumerb ; Wendie S. Cohicka ; cohick@aesop.rutgers.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：CTD ; C-terminal domain ; EGFP ; enhanced green fluorescent protein ; ER ; endoplasmic reticulum ; qRT-PCR ; quantitative reverse transcription PCR ; RIP ; ribosome-inactivating protein ; RTA ; ricin toxin A chain ; RTB ; ricin B-chain ; RU ; resonance units ; SRL ; sarcin-ricin loop ; Stx1 ; Shiga toxin 1 ; Stx2 ; Shiga toxin 2 ; TCS ; trichosanthin ; WT ; wild-type
- 刊名：Toxicology and Applied Pharmacology
- 出版年：2016
- 出版时间：1 November 2016
- 年：2016
- 卷：310
- 期：Complete
- 页码：120-128
- 全文大小：1054 K
文摘
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Arginines 193 and 235 of RTA are critical for binding to the mammalian ribosome.
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R193A/R235A has full catalytic activity on RNA but not on mammalian ribosomes.
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R193A/R235A is less toxic than a mutant that targets the active site.
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The toxin-ribosome interaction is a therapeutic target for ricin intoxication.