An in vitro evaluation of epigallocatechin gallate (eGCG) as a biocompatible inhibitor of ricin toxin
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- 作者：Paul D.R. Dyera ; 1 ; Arun K. Kothaa ; 1 ; Alex S. Gollingsa ; Susan A. Shortera ; Thomas R. Shepherda ; Marie W. Pettita ; Bruce D. Alexanderb ; Giulia T.M. Gettia ; Samer El-Dahera ; Les Bailliec ; Simon C.W. Richardsona ; S.C.W.Richardson@Greenwich.ac.uk" class="auth_mail" title="E-mail the corresponding author
- 关键词：BCA ; bicinchoninic acid ; BSA ; bovine serum albumin ; CD ; circular dichroism ; cRTAC ; commercially acquired RTAC ; cRTBC ; commercially acquired RTBC ; DMSO ; dimethyl sulfoxide ; ER ; endoplasmic reticulum ; eGCG ; epigallocatechin gallate ; FCS ; fetal calf serum ; MTT ; 3-(4 ; 5-dimethylthiazol-2-yl)-2 ; 5-diphenyltetrazolium bromide ; PMA ; 1-ethyl-3-(3-dimethylamino-propyl) phorbol 12-myristate-13-acetate ; rRTBC ; recombinant RTBC ; RT ; ricin toxin ; RTBC ; RT B chain ; RTAC ; RT A chain ; SEM ; standard error of the m
- 刊名：Biochimica et Biophysica Acta (BBA) - General Subjects
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：1860
- 期：7
- 页码：1541-1550
- 全文大小：1267 K
文摘
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Epigallocatechin gallate (eGCG) has inhibitory activity against the ricin toxin (RT).
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The reduction in ricin toxicity mediated by eGCG was concentration dependent.
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EGCG was found to reduce the binding of RT B chain to lactose-conjugated Sepharose.
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EGCG significantly reduces the uptake of RT B chain in Vero cells.
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EGCG may provide a starting point to refine substances that reduce ricin toxicity.