A nanoparticle-based bio-barcode assay for ultrasensitive detection of ricin toxin

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• 作者：Hui-qiong Yin^a ; Min-xian Jia^a ; Shu Yang^a ; Sheng-qi Wang^b ; Jin-gang Zhang^a ; ^{zhangjg@nic.bmi.ac.cn}
• 关键词：Ricin ; Bio-barcode assay ; Real-time PCR ; High-sensitivity detection
• 刊名：Toxicon
• 出版年：2012
• 出版时间：January 2012
• 年：2012
• 卷：59
• 期：1
• 页码：12-16
• 全文大小：336 K

文摘

The ultrasensitive bio-barcode amplification assay (BCA) technique was developed for the specific detection of the A chain of ricin toxin. The target antigen A chain was first captured by gold nanoparticles (GNPs) coated with polyclonal antibodies. Magnetic microparticles (MMPs) coated with A chain monoclonal antibody were then added to form a sandwich immuno-complex. After the immuno-complex was formed, signal DNA annealed to DNA strands covalently bound to the GNPs were released by heating and characterized by PCR and real-time fluorescence PCR. A detection limit of 1 fg/ml was measured for A chain, six orders of magnitude more sensitive than that of conventional antigen-capture ELISA. The coefficient of variation (CV) of intra-assay and inter-assay ranged from 3.39 % to 6.84 % . The BCA can detect the A chain in milk and water mimic samples. In the following work it is demonstrated that this assay is a highly sensitive method for the detection of ricin proteins that could be adapted to measure other proteins.