Therapeutic Angiogenesis Following Arterial Gene Transfer of Vascular Endothelial Growth Factor in a Rabbit Model of Hindlimb Ischemia

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• 作者：Takeshita ; Satoshi ; Weir ; Lawrence ; Chen ; Dongfen ; Zheng ; Lu P. ; Riessen ; Reimer ; Bauters ; Christophe ; et. al.
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：1996
• 出版时间：October 14, 1996
• 年：1996
• 卷：227
• 期：2
• 页码：628-635
• 全文大小：631 K

文摘

The plasmid phVEGF₁₆₅, expressing the 165-amino-acid isoform of vascular endothelial growth factor (VEGF), an endothelial cell specific mitogen, was applied to the polymer coating of an angioplasty balloon and delivered percutaneously to the iliac artery of rabbits in which the femoral artery had been excised to cause hindlimb ischemia. Site-specific transfection of phVEGF₁₆₅resulted in augmented development of collateral vessels documented by serial angiograms, and increased capillary density as well as increased capillary/myocyte ratio documented histochemically at necropsy. Consequent amelioration of the hemodynamic deficit in the ischemic limb was documented by improvement in the calf blood pressure ratio (ischemic/normal limb) to 0.70±0.08 in the VEGF-transfected group vs 0.50±0.18 in controls (p<0.05). These findings suggest that site-specific arterial gene transfer of VEGF₁₆₅may achieve physiologically meaningful therapeutic modulation of vascular insufficiency.