Development and validation of an UFLC-MS/MS method for enantioselectivity determination of d,l-thero-methylphenidate, d,l-thero-ethylphenidate and d,l-thero-ritalinic acid in rat plasma and its application to pharmacokinetic study
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- 作者：Chenghao Zhang ; Huafei Luo ; Yubo Wu ; Junyun Zhang ; Furong Zhang ; Guobei Lin ; Hao Wang ; 13162596759@163.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Methyphenidate ; Ethylphenidate ; Chiral separation ; UFLC-MS/MS ; Pharmacokinetics ; Drug interaction
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2016
- 出版时间：1 February 2016
- 年：2016
- 卷：1011
- 期：Complete
- 页码：45-52
- 全文大小：1378 K
文摘
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One step liquid–liquid extraction method to extract d-threo-MPH, l-threo-MPH, d-threo-EPH, l-threo-EPH and d,l-threo-RA was developed to replace the time-consuming SPE method.
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This study is the first UFLC-MS/MS method to quantify d-threo-MPH, l-threo-MPH, d-threo-EPH, l-threo-EPH and d,l-threo-RA simultaneously, and a better chiral separation of d-threo-MPH, l-threo-MPH, d-threo-EPH, l-threo-EPH was achieved by UFLC-MS/MS optimization.
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The concentrations of d-threo-MPH, l-threo-MPH, d-threo-EPH, l-threo-EPH and d,l-threo-RA in rat plasma were determined by the proposed UFLC-MS/MS method after oral administration of 2 mg/kg d-threo-MPH and l-threo-MPH as well as in combination in rats (n = 6).