Environmental enrichment induces neuroplastic changes in middle age female BalbC mice and increases the hippocampal levels of BDNF, p-Akt and p-MAPK1/2
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- 作者：G. Ram铆rez-Rodr铆guez ; M.A. Oca帽a-Fern谩ndez ; N.M. Vega-Rivera ; O.M. Torres-P茅rez ; A. G贸mez-S谩nchez ; E. Estrada-Camarena ; L. Ortiz-L贸pez
- 关键词：BDNF ; brain-derived neurotrophic factor ; CR ; calretinin ; DCX ; doublecortin ; EDTA ; ethylenediaminetetraacetic acid ; ENR ; environmental enrichment ; GCL ; granular cell layer ; NH ; normal housing ; PI3K ; phosphatidylinositol-3-kinase ; TTBS ; Tween 20-TBS ; ZT ; Zeitgeber time
- 刊名：Neuroscience
- 出版年：28 February, 2014
- 年：2014
- 卷：260
- 期：Complete
- 页码：158-170
- 全文大小：3063 K
文摘
Hippocampus is one of the brain regions in which neuroplastic changes occur. Paradigms such as environmental enrichment (ENR) have been used to prevent or delay the neuroplastic changes of the hippocampus during aging. Here, we investigated the beneficial effects of ENR on dendritic spines and hippocampal neurogenesis in middle age BalbC mice. ENR increased the number of dendritic spines, cell survival, and intermediate stages of the hippocampal neurodevelopment process. Also, ENR alters the distribution of cells involved in the neurogenic process along the dorsal-ventral dentate gyrus. In addition, ENR increased the proportion of cells with more mature dendritic morphology and net hippocampal neurogenesis. Whole-hippocampus protein extracts revealed that ENR increases the levels of BDNF, phospho-Akt and phospho-MAPK1/2, suggesting that the positive effects of ENR on neuroplasticity in middle age BalbC mice involve the participation of these key-signaling proteins. Our results suggest that ENR is a relevant strategy to prevent neuroplastic decline by increasing the formation of both dendritic spines and new neurons in the hippocampus during middle age.