Knockdown of nuclease activity in the gut enhances RNAi efficiency in the Colorado potato beetle, Leptinotarsa decemlineata, but not in the desert locust, Schistocerca gregaria
文摘
Non-specific DNA/RNA endonucleases or dsRNases are expressed and secreted in the digestive system of many insect species. Degradation of dsRNA by nucleases in the digestive system of insects contributes to a lower environmental RNAi efficiency. Also the highly environmental RNAi sensitive Colorado potato beetle possesses two dsRNases active in the gut lumen. Knockdown of the dsRNases in the gut of the Colorado potato beetle increases the sensitivity towards orally delivered dsRNA. A similar strategy in the desert locust proved unsuccessful, probably due to residual nuclease activity.