When transcription goes on Holliday: Double Holliday junctions block RNA polymerase II transcription in vitro

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• 作者：Anne Pipathsouk¹ ; Boris P. Belotserkovskii ; Philip C. Hanawalt ; ^{hanawalt@stanford.edu}
• 关键词：Holliday junctions ; Transcription ; Unusual DNA structures ; Repair ; Genomic instability
• 刊名：Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：1860
• 期：2
• 页码：282-288
• 全文大小：1041 K
• 卷排序：1860

文摘

Holliday junctions cause RNAPII transcription arrest in HeLa cell nuclear extracts. Double Holliday junctions have stronger RNAPII blockage than single junctions. Holliday junctions do not produce detectable blockage for purified T7 RNAP. We discuss propose topological constraints as the mechanism for transcription blockage. Holliday junctions are implicated as novel structures to block transcription.