Production of infectious salmon anaemia virus (ISAV) ribonucleoprotein complexes using a mammalian cell based minigenome system
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- 作者：Mickael C.S. Fourrier ; Mickael.Fourrier@gov.scot ; Bertrand Collet Bertrand.Collet@gov.scot
- 关键词：CPE ; cytopathic effect ; F ; fusion protein ; FF ; firefly luciferase ; FFU ; focus forming units ; GFP ; green fluorescence protein ; HE ; Haemagglutinin-esterase ; Pol I/II ; polymerase I/II ; pPol I- FF ; plasmid with a polymerase I promoter and firefly luciferase reporter gene ; pPol I- GFP ; plasmid with a polymerase I promoter and green fluorescence protein reporter gene ; RLU ; Relative light units ; RNP ; ribonucleoprotein ; UTR ; untranslated region ; VLP ; virus-like particle ; vRNA ; viral ribonucleic acid
- 刊名：Journal of Virological Methods
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：239
- 期：Complete
- 页码：75-82
- 全文大小：1165 K
- 卷排序：239
文摘
Demonstration that ISAV replicates and produces functional virions in BHK-21 cells. Production of ISAV recombinant vRNA in BHK-21 cells using a plasmid with a Mouse Pol I promoter/terminator sequences. Production of functional RNPs expressing GFP and FF luciferase. Specific inhibition of RNPs polymerase transcription by rivabirin treatment.