Localization of histamine H<sub>4sub> receptors in the central nervous system of human and rat

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• 作者：Marina I. Strakhova ; Arthur L. Nikkel ; Arlene M. Manelli ; Gin C. Hsieh ; Timothy A. Esbenshade ; Jorge D. Brioni ; Robert S. Bitner
• 关键词：Histamine H<sub>4sub> receptor ; RT-PCR ; Immunohistochemistry ; Brain ; Dorsal root ganglia ; Spinal cord
• 刊名：Brain Research
• 出版年：2009
• 出版时间：23 January 2009
• 年：2009
• 卷：1250
• 期：Complete
• 页码：41-48
• 全文大小：3113 K

文摘

Existing data on the expression of H<sub>4sub> histamine receptor in the CNS are conflicting and inconclusive. In this report, we present the results of experiments that were conducted in order to elucidate H<sub>4sub> receptor expression and localization in the brain, spinal cord, and dorsal root ganglia (DRG). Here we show that transcripts of H<sub>4sub> receptor are present in all analyzed regions of the human CNS, including spinal cord, hippocampus, cortex, thalamus and amygdala, with the highest levels of H<sub>4sub> mRNA detected in the spinal cord. In rat, H<sub>4sub> mRNA was detected in cortex, cerebellum, brainstem, amygdala, thalamus and striatum. Very low levels of H<sub>4sub> mRNA were detected in hypothalamus, and no H<sub>4sub> signal was detected in the rat hippocampus. Fairly low levels of H<sub>4sub> mRNA were detected in examined peripheral tissues including spleen and liver. Interestingly, strong expression of H<sub>4sub> mRNA was detected in the rat DRG and spinal cord. Immunohistochemical analysis revealed expression of H<sub>4sub> receptors on neurons in the rat lumbar DRG and in the lumbar spinal cord. Our observations provide evidence of the H<sub>4sub> presence in both human and rodent CNS and offer some insight into possible role of H<sub>4sub> in itch and pain.