- 作者：Roberto Ghiselli ; M.D.&lowast ; ; robertoghiselli@hotmail.it ; Guendalina Lucarini ; Ph.D.&dagger ; ; Alessandra Filosa ; M.D.&Dagger ; ; Daniele Minardi ; M.D.§ ; ; Giuseppe Pelliccioni ; M.D.¶ ; ; Fiorenza Orlando ; Ph.D.?/sup> ; Roberto di Primio ; M.D.&dagger ; ; Rodolfo Montironi ; M.D.&Dagger ; ; Giovanni Muzzonigro ; M.D.§ ; ; Mario Guerrieri ; M.D.&lowast ;
- 关键词：neuromodulation ; animal model ; anorectal diseases ; nitric oxide
- 刊名：Journal of Surgical Research
- 出版年：2012
- 出版时间：July, 2012
- 年：2012
- 卷：176
- 期：1
- 页码：29-33
- 全文大小：904 K
Background
Sacral neuromodulation is becoming established as a valid treatment option for patients with anorectal disorders. Nevertheless, despite its efficacy, little is known regarding its mechanism of action. The purpose of this study was to evaluate whether chronic sacral neuromodulation is able to influence the expression of nitric oxide synthetase (NOS) in the anorectum of rats.Materials and methods
Twenty-six female Sprague-Dawley rats were divided into three groups; normal control rats (n = 6); sham treatment (n =10) and group in whom, electrical sacral neuromodulation was performed (n = 10). Bilateral electrode wires were placed in the S1 and electrical stimulation was performed for 14 d. At the end of the procedures the rats were sacrificed, proctectomy was performed, and anorectal specimens were sent to the laboratory for immunostaining with n-NOS and i-NOS.
Results
In the anal and rectal specimens, n-NOS and i-NOS expression was significantly increased in epithelial and muscle cells after neuromodulation of the anus and rectum of the animals.
Conclusion
Our results showed that this model can be applied in further experimental studies to better understand the mechanism of action of sacral neuromodulation in anorectal disorders.