BMAP-28 improves the efficacy of vancomycin in rat models of gram-positive cocci ureteral stent infection

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• 作者：Fiorenza Orl ; o ; Roberto Ghiselli ; Oscar Cirioni ; Daniele Minardi ; Linda Tomasinsig ; Federico Mocchegiani ; Carmela Silvestri ; Barbara Skerlavaj ; Aless ; ra Riva ; Giovanni Muzzonigro ; Vittorio Saba ; Giorg
• 关键词：Ureteral stent ; Biofilm ; Cathelicidins ; Animal model ; Gram-positive cocci
• 刊名：Peptides
• 出版年：2008
• 出版时间：July 2008
• 年：2008
• 卷：29
• 期：7
• 页码：1118-1123
• 全文大小：163 K

文摘

An experimental study was performed to evaluate the efficacy of BMAP-28 alone and in combination with vancomycin in animal models ureteral stent infection due to Enterococcus faecalis and Staphylococcus aureus. Study included a control group without bacterial challenge to evaluate the sterility of surgical procedure, a challenged control group that did not receive any antibiotic prophylaxis and for each bacterial strain three challenged groups that received (a) 10 mg/kg vancomycin intraperitoneally, immediately after stent implantation, (b) BMAP-28-coated ureteral stents where 0.2-cm² sterile ureteral stents were incubated in 1 mg/l BMAP-28 solution for 30 min immediately before implantation and (c) intraperitoneal vancomycin plus BMAP-28-coated ureteral stent at the above concentrations. Experiments were performed in duplicate. Ureteral stents were explanted at day 5 following implantation and biofilm bacteria enumerated. Our data showed that rats that received intraperitoneal vancomycin showed the lowest bacterial numbers. BMAP-28 combined with vancomycin showed efficacies higher than that of each single compound. These results highlight the potential usefulness of this combination in preventing ureteral stent-associated in gram-positive infections.