Comparison of Low-Fat Versus Mediterranean-Style Dietary Intervention After First Myocardial Infarction (from The Heart Institute of Spokane Diet Intervention and Evaluation Trial)
详细信息 查看全文
- 作者：Katherine R. Tuttle ; Lynn A. Shuler ; Diane P. Packard ; Joan E. Milton ; Kenn B. Daratha ; Douglas M. Bibus ; Robert A. Short
- 关键词：Magnetoliposomes ; Autoantibodies ; Colloidal magnetite ; Immunoglobulin binding
- 刊名：The American Journal of Cardiology
- 出版年：2008
- 出版时间：1 June 2008
- 年：2008
- 卷：101
- 期：11
- 页码：1523-1530
- 全文大小：344 K
文摘
This work describes the preparation, characterization and in vitro adsorption tests of surface-modified magnetoliposomes for affinity binding of (i) anticardiolipin (isotype G) antibodies and (ii) specific isotype E antibodies generated by hypersensitivity reactions in humans with respiratory allergy. In the first case, cardiolipin embedded in the bilayer of magnetoliposomes was used as specific ligand. In the second case, antigenic proteins present in an extract of Dermatophagoids pteronyssinus and Blomia tropicalis mites were covalently coupled on the surface of magnetoliposomes via a diglycolic spacer arm, and used as specific ligands for IgE. Antibody adsorption was performed in a high-gradient magnetophoresis system, using either sera of healthy individuals or a pool of sera from autoimmune or allergic patients. The selectivity and capacity of the system were quantified by a frontal analysis in a capillary column, and by constructing breakthrough curves. The results show that the highest yield and selectivity were obtained if the ligand was extended into the aqueous layer surrounding the magnetoliposome surface. A 100 % selectivity was obtained for adsorption of specific IgE, and 8 % for IgG. These results demonstrate the potentialities of both types of surface-modified magnetic biocolloids in the field of in vitro diagnosis tests for allergic or autoimmune conditions.