A new Hu-PBL model for the study of human islet alloreactivity based on NOD-scid mice bearing a targeted mutation in the IL-2 receptor gamma chain gene

详细信息    查看全文

• 作者：Marie King ; Todd Pearson ; Leonard D. Shultz ; Jean Leif ; Rita Bottino ; Massimo Trucco ; Mark A. Atkinson ; Clive Wasserfall ; Kevan C. Herold ; Robert T. Woodl ; Madelyn R. Schmidt ; Bruce A. Woda ; Michael
• 关键词：NOD-scid ; IL-2r gamma chain ; Humanized mice ; SCID ; Transplantation ; Islet ; Rejection ; Hu-PBL-SCID ; Human ; Mouse
• 刊名：Clinical Immunology
• 出版年：2008
• 出版时间：March 2008
• 年：2008
• 卷：126
• 期：3
• 页码：303-314
• 全文大小：1788 K

文摘

Natural Tregs play an essential role in controlling self-tolerance but the in vivo sites of Treg-mediated suppression remain controversial. We have previously reported the identification of distinct naïve and memory Treg populations in human circulating lymphocytes. Here we show that memory Tregs contain high proportions of inflammatory chemokine-expressing cells and comprise two populations that differ in the expression of the lymphoid chemokine receptor CCR7 and represent the counterparts of conventional CCR7⁺ central memory (CM) and CCR7⁻ effector memory (EM) T cells. CM and EM Tregs exert comparable ex vivo suppressor functions but the EM population is more prominent among Tregs as compared to conventional CD4⁺ T cells, and is the main Treg subset found in ovarian tumors. Our data suggest that a division of labor between CM and EM Tregs ensures tolerance at lymphoid and peripheral locations including tumor sites.