Detection of Streptococcus equi subspecies equi using a triplex qPCR assay

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• 作者：Katy Webb¹ ; Colin Barker¹ ; Tihana Harrison ; Zoe Heather ; Karen F. Steward ; Carl Robinson ; J. Richard Newton ; Andrew S. Waller ; ^{andrew.waller@aht.org.uk}
• 关键词：Equine ; Strangles ; Streptococcus equi ; Triplex quantitative PCR
• 刊名：The Veterinary Journal
• 出版年：2013
• 出版时间：March, 2013
• 年：2013
• 卷：195
• 期：3
• 页码：300-304
• 全文大小：388 K

文摘

Genome sequencing data for Streptococcus equi subspecies equi and zooepidemicus were used to develop a novel diagnostic triplex quantitative PCR (qPCR) assay targeting two genes specific to S. equi (eqbE and SEQ2190) and a unique 100 base pair control DNA sequence (SZIC) inserted into the SZO07770 pseudogene of S. zooepidemicus strain H70. This triplex strangles qPCR assay can provide results within 2 h of sample receipt, has an overall sensitivity of 93.9 % and specificity of 96.6 % relative to the eqbE singlex assay and detects S. equi at levels below the threshold of the culture assay, even in the presence of contaminating bacteria.