The anti-inflammatory effects of N-methyl-(2S,4R)-trans-4-hydroxy-l-proline from Syderoxylon obtusifolium are related to its inhibition of TNF-alpha and inflammatory enzymes

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• 作者：Pedro Everson Alexandre de Aquino^a ; Talita Rocha Magalhã ; es^a ; Lucas Antô ; nio Duarte Nicolau^a ; Luzia Kalyne A. Moreira Leal^a ; Nayara Coriolano de Aquino^b ; Sabrina Matias dos Santos^b ; Kelly Rose Tavares Neves^a ; Edilberto Rocha Silveira^b ; Glauce Socorro de Barros Viana^a ; ^c ; ^{gbviana@live.com}
• 关键词：Sideroxylon obtusifolium ; Inflammation ; Cytokines ; Oxidative stress
• 刊名：Phytomedicine
• 出版年：2017
• 出版时间：15 January 2017
• 年：2017
• 卷：24
• 期：Complete
• 页码：14-23
• 全文大小：4269 K
• 卷排序：24

文摘

Sideroxylon obtusifolium (Roem. & Schult.) T.D. Penn., Sapotaceae family, is a medicinal species native to the Brazilian Northeastern region. The plant is popularly used as an anti-inflammatory and hypoglycemic.PurposeTo evaluate the anti-inflammatory properties of the N-methyl-(2S,4R)-trans-4-hydroxy-l-proline (NMP) from S. obtusifolium leaves in models of inflammation and to clarify its action mechanisms.MethodsMale Swiss mice were distributed intocontrols and groups treated with NMP (25, 50 and 100 mg/kg, p.o.), indomethacin or morphine (reference drugs). The animals were subjected to the formalin, carrageenan-induced edema and peritonitis tests. Furthermore, peritoneal lavage and slices from edematous paws were used for histological and immunohistochemical (iNOS, TNF-alpha, COX-2 and NF-kB) assays.ResultsDecreases in licking time, in the 1st and mainly in the 2nd phases of the formalin test, were shown after NMP treatments. In addition, decreases (around 50%) in paw edema were noticed at the 3rd h. The HE staining of paw slices demonstrated a complete reversion of the increased PMN cell numberafter NMP treatment. Similarly, decreases higher than 70% were also demonstrated in PMN cells, in the peritoneal fluid. Furthermore, NMP significantly decreased iNOS, TNF-alpha, COX-2 and NF-kB immunoreactivities.ConclusionsWe showed that S. obtusifolium presents a potent anti-inflammatory activity, due to the presence of the N-methyl-(2S,4R)-trans-4-hydroxy-l-proline(NMP) in the plant extract. This action is related to the inhibition by NMP of TNF-alpha and inflammatory enzymes.