dsRNA-protein interactions studied by molecular dynamics techniques. Unravelling dsRNA recognition by DCL1
详细信息 查看全文
- 作者：Salvador I. Drusina ; b ; Irina P. Suareza ; Diego F. Gautoa ; Rodolfo M. Rasiaa ; c ; Diego M. Morenod ; e ; moreno@iquir-conicet.gov.ar" class="auth_mail" title="E-mail the corresponding author
- 关键词：Molecular dynamics ; dsRNA recognition ; dsRBD ; miRNA processing ; Binding free energy ; Mismatch base pair
- 刊名：Archives of Biochemistry and Biophysics
- 出版年：2016
- 出版时间：15 April 2016
- 年：2016
- 卷：596
- 期：Complete
- 页码：118-125
- 全文大小：1627 K
文摘
- class="listitem" id="list_ulist0010">
- class="label">•
We characterized with atomic detail the binding of DCL1-1 to substrate dsRNA.
- class="label">•
DCL1-1 induces an axial bend in the dsRNA helix.
- class="label">•
Arg8 plays a key role in the recognition of the dsRNA sequence.
- class="label">•
Arg8 interacts preferentially with non-canonical base-pairs.
- class="label">•
Mismatches could be the structural key for pri-miRNA recognition by DCL1-1.