Comparison of two chemiluminescent immunoassays in the detection of measles IgM antibodies
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- 作者：Cristina Gó ; mez-Camarasa gomezcamarasa@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Ana Lara-Oya ana.lara.sspa@juntadeandalucia.es" class="auth_mail" title="E-mail the corresponding author ; Fernando Cobo fernando.cobo.sspa@juntadeandalucia.es" class="auth_mail" title="E-mail the corresponding author ; Antonio Sampedro-Martí ; nez ; antonioj.sampedro.sspa@juntadeandalucia.es" class="auth_mail" title="E-mail the corresponding author ; Javier Rodrí ; guez-Granger javierm.rodriguez.sspa@juntadeandalucia.es" class="auth_mail" title="E-mail the corresponding author ; José ; Gutierrez-Ferná ; ndez josegf@ugr.es" class="auth_mail" title="E-mail the corresponding author ; José ; Marí ; a Navarro-Marí ; josem.navarro.sspa@juntadeandalucia.es" class="auth_mail" title="E-mail the corresponding author
- 关键词：Measles ; Serology ; Chemiluminescent immunoassay
- 刊名：Journal of Virological Methods
- 出版年：2016
- 出版时间：November 2016
- 年：2016
- 卷：237
- 期：Complete
- 页码：38-39
- 全文大小：178 K
文摘
Serological confirmation of measles is achieved by detecting the specific immunoglobulin M (IgM), and it is important to evaluate new commercial inmunoassays in order to ensure the quality of results. The objective of this study was to compare the performance of a novel automated chemiluminescent immunoassay (CLIA), Virclia IgM measles (Vircell, Spain), with that of the widely used Liaison measles IgM assay (DiaSorin, Italy). A panel of 86 sera from laboratory-confirmed cases was used for the sensitivity calculation, and 59 sera from healthy individuals and those with other viral infections were used for the specificity calculation. Sensitivity values were 96.5% for Virclia and 97.6% for Liaison; specificity values were 93.2% for Virclia and 96.6% for Liaison; neither difference was statistically significant VirClia IgM measles is a good alternative to other immunoassays for the serological confirmation of measles.