Oxidative Stress in Brain According to Traumatic Brain Injury Intensity

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• 作者：Fabricia Petronilho ; Gustavo Feier ; Bruna de Souza ; Cecí ; lia Guglielmi ; Larissa S. Constantino ; Roger Walz ; Joã ; o Quevedo ; Felipe Dal-Pizzol
• 关键词：traumatic brain injury ; lipid peroxidation ; protein carbonyls
• 刊名：Journal of Surgical Research
• 出版年：2010
• 出版时间：December 2010
• 年：2010
• 卷：164
• 期：2
• 页码：316-320
• 全文大小：338 K

文摘

Background

The mechanisms of brain damage and neuroplasticity following traumatic brain injury (TBI) are complex and not completely understood. Thus, we investigated markers of oxidative stress in the central nervous system after mild and severe TBI in rats.

Material and Methods

Adult male wistar rats (five animals per group) submitted to mild (mTBI group) or severe TBI (sTBI Group) were sacrificed 30min, 3, 6, or 12 h after the injury to quantify markers of oxidative damage in different brain regions. Levels of thiobarbituric acid reactive species and protein carbonyl in the cortex, hippocampus, striatum, and cerebellum of mTBI and sTBI groups were compared with the control group.

Results

After mTBI, levels of protein oxidation were increased in all analyzed structures in several different times after injury. The increase in TBARS levels was not so consistent in mTBI. In contrast, sTBI did not induce a sustainable increase in oxidative damage markers in all analyzed structures.

Conclusions

Oxidative damage seemed to be inversely proportional to severity of traumatic brain injury.