Phylogenetic analysis of enteric species of the family Enterobacteriaceae using the oriC-locus

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• 作者：Andreas Roggenkamp
• 关键词：Oric ; Enteric species ; Enterobacteriaceae ; Coliform group ; Phylogenetic study
• 刊名：Systematic and Applied Microbiology
• 出版年：2007
• 出版时间：19 April 2007
• 年：2007
• 卷：30
• 期：3
• 页码：180-188
• 全文大小：200 K

文摘

Phylogenetic analysis of 38 enteric species belonging to the Enterobacteraceae family was carried out using the non-coding locus oriC, the chromosomal replication origin. The oriC loci were amplified with conserved oligonucleotides and the PCR fragments were sequenced directly. The results establish a phylogenetic tree for the classification of different species of the genera Escherichia, Shigella, Salmonella, Enterobacter, Citrobacter, Klebsiella, Raoultella, Kluyvera, Cedecea and Buttiauxella. Functional important protein-binding sites located in oriC are well conserved throughout the enteric group. More over, due to a high overall divergence value phylogenetic trees were robust and well supported by bootstrap analysis. In comparison with 16S rDNA analysis, the oriC sequences indicated a greater evolutionary divergence for bacteria. We propose that the oriC locus might be a suitable phylogenetic marker for the identification and classification of bacteria, in particular for closely related species.