E. coli

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• 作者：Assaf Rokney ; Merav Shagan ; Martin Kessel ; Yoav Smith ; Ilan Rosenshine ; Amos B. Oppenheim
• 关键词：protein aggregation ; protein quality control ; bacteria ; pole ; polar localization
• 刊名：Journal of Molecular Biology
• 出版年：2009
• 出版时间：25 September 2009
• 年：2009
• 卷：392
• 期：3
• 页码：589-601
• 全文大小：715 K

文摘

Aggregation of proteins due to failure of quality control mechanisms is deleterious to both eukaryotes and prokaryotes. We found that in Escherichia coli, protein aggregates are delivered to the pole and form a large polar aggregate (LPA). The formation of LPAs involves two steps: the formation of multiple small aggregates and the delivery of these aggregates to the pole to form an LPA. Formation of randomly distributed aggregates, their delivery to the poles, and LPA formation are all energy-dependent processes. The latter steps require the proton motive force, activities of the DnaK and DnaJ chaperones, and MreB. About 90 min after their formation, the LPAs are dissolved in a process that is dependent upon ClpB, DnaK, and energy. Our results confirm and substantiate the notion that the formation of LPAs allows asymmetric inheritance of the aggregated proteins to a small number of daughter cells, enabling their rapid elimination from most of the bacterial population. Moreover, the results show that the processing of aggregated proteins by the protein quality control system is a multi-step process with distinct spatial and temporal controls.