ORGANIZATION OF FOCAL ADHESION PLAQUES IS DISRUPTED BY ACTION OF THE HIV-1 PROTEASE

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• 作者：Shoeman ; Robert L. ; Hartig ; Roland ; Hauses ; Claudia ; Traub ; Peter
• 关键词：focal adhesion plaque ; HIV-1 protease ; vinculin ; vimentin ; focal adhesion plaque kinase ; CLSM ; confocal laser scanning microscopy ; ECM ; extracellular matrix ; FAK ; focal adhesion plaque kinase ; HIV-1 ; human immunodeficiency virus type 1 ; HIV-1 PR ; wild-typ
• 刊名：Cell Biology International
• 出版年：2002
• 出版时间：June, 2002
• 年：2002
• 卷：26
• 期：6
• 页码：529-539
• 全文大小：309 K

文摘

Focal adhesion plaques were severely affected in human embryonic fibroblasts permeabilized with digitonin and incubated in buffer containing the human immunodeficiency virus type 1 protease (HIV-1 PR). A mutant HIV-1 PR (3271 HIV-1 PR) had no effect on focal adhesion plaques. Similar effects were seen with cells microinjected with either HIV-1 PR or 3271 HIV-1 PR. Immunoblots of the human embryonic fibroblasts demonstrated that a number of focal adhesion plaque proteins were specifically cleaved by HIV-1 PR. These included fimbrin, focal adhesion plaque kinase (FAK), talin, and, to a lesser extent, filamin, spectrin and fibronectin. Proteins detected by antibodies to β4 integrin and α3 integrin were also cleaved by the HIV-1 PR. Control experiments demonstrated that the effect and protein cleavages described are due to action of the HIV-1 PR and not to the action of endogenous host cell proteases.