THO/Sub2p Functions to Coordinate 3′-End Processing with Gene-Nuclear Pore Association

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• 作者：Mathieu Rougemaille ; Guennaelle Dieppois ; Elena Kisseleva-Romanova ; Rajani Kanth Gudipati ; Sophie Lemoine ; Corinne Blugeon ; Jocelyne Boulay ; Torben Heick Jensen ; Franç ; oise Stutz ; Fré ; dé ; r
• 刊名：Cell
• 出版年：2008
• 出版时间：17 October 2008
• 年：2008
• 卷：135
• 期：2
• 页码：308-321
• 全文大小：1132 K

文摘

Summary

During transcription, proteins assemble sequentially with nascent RNA to generate a messenger ribonucleoprotein particle (mRNP). The THO complex and its associated Sub2p helicase are functionally implicated in both transcription and mRNP biogenesis but their precise function remains elusive. We show here that THO/Sub2p mutation leads to the accumulation of a stalled intermediate in mRNP biogenesis that contains nuclear pore components and polyadenylation factors in association with chromatin. Microarray analyses of genomic loci that are aberrantly docked to the nuclear pore in mutants allowed the identification of approximately 400 novel validated target genes that require THO /Sub2p for efficient expression. Our data strongly suggests that the THO complex/Sub2p function is required to coordinate events leading to the acquisition of export competence at a step that follows commitment to 3′-processing.