α-Phellandrene, a cyclic monoterpene, attenuates inflammatory response through neutrophil migration inhibition and mast cell degranulation

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• 作者：Halmisson D'Arley S. Siqueira^a ; ^c ; Benedito S. Neto^a ; ^c ; Damiã ; o P. Sousa^b ; ^c ; Bruno S. Gomes^a ; ^c ; Francilene Vieira da Silva^a ; ^c ; Francisco V.M. Cunha^a ; ^c ; ^{orfeuyeuridice@yahoo.com.br" class="auth_mail" title="E-mail the corresponding author} ; Carlos W.S. Wanderley^b ; ^c ; Gabriel Pinheiro^b ; ^c ; André ; G.F. Câ ; ndido^b ; ^c ; Deysi V.T. Wong^b ; ^c ; Ronaldo A. Ribeiro^b ; ^c ; ¹ ; Roberto C.P. Lima-Jú ; nior^b ; ^c ; Francisco A. Oliveira^a ; ^c
• 关键词：α-Phellandrene ; Inflammation ; Peritonitis ; Air-pouch ; Neutrophil ; Mast cell
• 刊名：Life Sciences
• 出版年：2016
• 出版时间：1 September 2016
• 年：2016
• 卷：160
• 期：Complete
• 页码：27-33
• 全文大小：1021 K

文摘

We aimed to investigate the modulating effect of α-phellandrene on neutrophil migration and mast cell degranulation processes.

Main methods

Male Wistar rats or Swiss mice were treated p.o. with vehicle (3% Tween 80, p.o.), α-phellandrene (50, 100, or 200 mg/kg, p.o.), or dexamethasone (0.5 mg/kg, p.o.) 1 h before carrageenan injection. Then, the neutrophil migration in 6-day-old air pouches or peritoneal cavities. The leukocyte rolling and adhesion were measured in real time and assessed by intravital microscopy. ELISA was used to detect TNF-α and IL-6 in peritoneal lavage. Compound 48/80–induced mast cell degranulation was assessed in mesenteric rat tissues.

Key findings

In all the tested doses, α-phellandrene prevented carrageenan-induced neutrophil accumulation (P < 0.05). As detected by intravital microscopy, α-phellandrene also inhibited leukocyte rolling and adhesion, as well as significantly inhibited the production of the pro-inflammatory cytokines TNF-α and IL-6. Moreover, the degranulation of compound 48/80–induced mast cells was also inhibited by α-phellandrene (P < 0.001).

Significance

These results suggest that α-phellandrene plays an important role as an anti-inflammatory agent through neutrophil migration modulation and mast cell stabilization.