Simultaneous determination of anemoside B4, phellodendrine, berberine, palmatine, obakunone, esculin, esculetin in rat plasma by UPLC-ESI-MS/MS and its application to a comparative pharmacokinetic study in normal and ulcerative colitis rats
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- 作者：Lianrong Yang ; lianrong_790717@sina.com ; Xin Meng ; Xiaojin Yu ; Haixue Kuang
- 关键词：PD ; Pulsatillae Decoction ; UPLC&ndash ; ESI&ndash ; MS/MS ; ultra-performance liquid chromatography&ndash ; electrospray ionization&ndash ; mass spectrometry ; UC ; ulcerative colitis ; MRM ; multiple reaction monitoring
- 刊名：Journal of Pharmaceutical and Biomedical Analysis
- 出版年：2017
- 出版时间：5 February 2017
- 年：2017
- 卷：134
- 期：Complete
- 页码：43-52
- 全文大小：2428 K
- 卷排序：134
文摘
Detection and measurement were performed on a 4000 QTRAP UPLC–MS/MS system from AB Sciex in the multiple reaction monitoring (MRM) mode. A quantitative UPLC–MS/MS method was developed for the simultaneous determination of seven analytes. Compare the pharmacokinetics of seven active ingredients in normal and ulcerative colitis rats. The analytical results demonstrated the quantification of seven analytes without interferences from the endogenous matrix.