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Two novel pH probes (rong class="boldFont">L1rong> and rong class="boldFont">L2)rong> were facilely synthesized by one-step reaction.
Fluorescence intensity ratio of rong class="boldFont">L1rong> and rong class="boldFont">L2rong> can quantify the pH fluctuations.
rong class="boldFont">L1rong> and rong class="boldFont">L2rong> show high quantum yield and large two-photon absorption cross-section.
rong class="boldFont">L1rong> and rong class="boldFont">L2rong> show excellent selectivity response and remarkable color change to H+.
rong class="boldFont">L1rong> and rong class="boldFont">L2rong> were successfully utilized to mapping pH difference intracellularly.
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