Urinary nitrite/nitrate ratio measured by isotope-dilution LC-MS/MS as a tool to screen for urinary tract infections
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- 作者：Mu-Rong Chaoa ; Ying-Ming Shihb ; c ; Yu-Wen Hsua ; d ; Hung-Hsin Liua ; Yuan-Jhe Changc ; Bo-Huei Lina ; Chiung-Wen Huc ; e ; cwhu0823@gmail.com" class="auth_mail" title="E-mail the corresponding author ; windyhu@csmu.edu.tw" class="auth_mail" title="E-mail the corresponding author
- 关键词：ESI ; electrospray ionization ; LC&ndash ; MS/MS ; liquid chromatography&ndash ; tandem mass spectrometry ; LOD ; limit of detection ; LOQ ; limit of quantification ; NO ; nitric oxide ; SPE ; solid-phase extraction ; UTI ; urinary tract infection
- 刊名：Free Radical Biology and Medicine
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：93
- 期：Complete
- 页码：77-83
- 全文大小：636 K
文摘
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We developed a LC-MS/MS method to measure nitrite and nitrate in human urine.
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Derivatization of nitrite and enzymatic reduction of nitrate were optimized.
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UTI patients had significantly higher urinary ratio of nitrite to nitrate.
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The nitrite/nitrate ratio had significantly decreased after antibiotic treatment.
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The nitrite/nitrate ratio is a better method for UTIs diagnosis than nitrite alone.