Tanshinone IIA protects neonatal rat cardiomyocytes from adriamycin-induced apoptosis

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• 作者：Jie Gao ; Guoqing Yang ; Rongbiao Pi ; Ruifang Li ; Ping Wang ; Huijie Zhang ; Kang Le ; Shaorui Chen ; Peiqing Liu
• 刊名：Translational Research
• 出版年：2008
• 出版时间：February 2008
• 年：2008
• 卷：151
• 期：2
• 页码：79-87
• 全文大小：718 K

文摘

Tanshinone IIA (TSN) is a monomer extracted from the Chinese herb Danshen. In this study, we examined the effect of Tanshinone IIA on adriamycin (ADR)-induced apoptosis in neonatal rat cardiomyocytes and underlying molecular mechanisms. Primary cultured cardiomyocytes were treated with 1 μmol/L of adriamycin for 24 h with or without pretreatment with Tanshinone IIA (0.5–2 μmol/L) for 2 h. 3-(4,5-dimethyl thiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay, Hoechst staining, and flow cytometry measurement were used to assess cell viability and apoptosis. Fluorescent probes 2’,7’-dichlorofluorescein diacetate and dihydroethidium were used to detect the production of reactive oxygen species. Western blotting was used to evaluate the expression of Bcl-2 and Bax proteins. Adriamycin significantly induced apoptosis in cardiomyocytes. Tanshinone IIA (0.5–2 μmol/L) ameliorated apoptosis induced by adriamycin in a dose-dependent manner. Tanshinone IIA (2 μmol/L) markedly attenuated adriamycin-induced reactive oxygen species production. Western blotting revealed that Tanshinone IIA prevented the adriamycin-mediated reduction of the ratio of Bcl-2/Bax. In conclusion, Tanshinone IIA significantly inhibits adriamycin-induced cardiomyocyte apoptosis in a dose-dependent manner, and this effect is at least partly caused by its antioxidant properties.