Enhanced expression of vascular cell adhesion molecule-1 by corticotrophin-releasing hormone contributes to progression of atherosclerosis in LDL receptor-deficient mice

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• 作者：Yuqing Wu ; Rongjian Zhang ; Chenghua Zhou ; Youhua Xu ; Xiaowei Guan ; Jue Hu ; Yinyan Xu ; Shengnan Li
• 关键词：Corticotrophin-releasing hormone ; Atherosclerosis ; Inflammation ; Cell adhesion molecule ; Transcription factor
• 刊名：Atherosclerosis
• 出版年：2009
• 出版时间：April 2009
• 年：2009
• 卷：203
• 期：2
• 页码：360-370
• 全文大小：1853 K

文摘

Peripherally produced corticotrophin-releasing hormone (CRH) is a strong proinflammatory factor involved in many inflammatory diseases. However, to date, there is no evidence about the action of CRH on atherosclerosis, a chronic disease characterized by inflammatory reactions. In this study we observed the effect of CRH on atherosclerosis in low-density lipoprotein receptor-deficient (LDLr−/−) mice. Twelve-week-old, male LDLr−/− mice were subcutaneously injected with CRH (10 μg/kg) or vehicle once a day for 8 weeks. The results indicated aortic atherosclerotic lesions were larger (P < 0.01) in CRH-treated mice than those in untreated mice. CRH significantly up-regulated the expression of both protein and mRNA for vascular cell adhesion molecule-1 (VCAM-1), together with a markedly increased activation of nuclear factor kappa B (NF-κB) in aortas. In addition, the blood lipid levels were not influenced by CRH subcutaneous injection. The significant proatherogenic effect of CRH in LDLr−/− mice was largely attenuated by selective CRH receptor 1 (CRHR1) antagonist NBI27914 but not by specific CRH receptor 2 (CRHR2) antagonist antisauvagine-30 (anti-Svg-30). Meanwhile, both the enhanced expression of VCAM-1 and increased activation of NF-κB induced by CRH in aortas of LDLr−/− mice were also largely suppressed by NBI27914, whereas these inhibitory effects were not observed in anti-Svg-30 group. Taken together, these findings indicated that CRH may accelerate atherosclerosis progression in LDLr−/− mice via CRHR1. The enhanced VCAM-1 expression which probably resulted from increased activation of NF-κB induced by CRH, may be one of the important molecular mechanisms by which CRH accelerates atherosclerosis. This study provides a new insight into the effect of CRH on atherosclerosis and suggests a potential target for the prevention and treatment of atherosclerosis.