Flow-cytometric analysis of reactive oxygen species in cancer cells under treatment with brassinosteroids
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- 作者：Pyotr A. Kisselev ; Olesya V. Panibrat ; Aliaksei R. Sysa ; Marina V. Anisovich ; Vladimir N. Zhabinskii ; vz@iboch.bas-net.by ; Vladimir A. Khripach
- 关键词：BS ; brassinosteroids ; DCF ; 2&prime ; 7&prime ; -dichlorofluorescein ; DCFH-DA ; 2&prime ; 7&prime ; -dichlorodihydrofluorescein diacetate ; DMEM ; Dulbecco&rsquo ; s modified Eagle&rsquo ; s medium ; EBl ; 24-epibrassinolide ; Et-Br ; ethidium bromide ; FBS ; fetal bovine serum ; 28-homocastasterone ; 28-homoCS ; (22S ; 23S)-28-homocastasterone ; (22S ; 23S)-28-homoCS ; MTT ; 3-(4 ; 5-dimethyltiazol-2-yl)-2 ; 5-diphenyltetrazolium bromide ; PBS ; phosphate-buffered saline ; RFU ; relative fluorescence units ; ROS ; reactive oxygen spe
- 刊名：Steroids
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：117
- 期：Complete
- 页码：11-15
- 全文大小：909 K
- 卷排序：117
文摘
Brassinosteroids induced generation of reactive oxygen species (ROS) in A549 cells. The maximal effect was observed for (22S,23S)-28-homocastasterone. It exhibited a 6-fold increase of ROS generation at 30 μM concentration.