Cancer biomarker and neurotransmitters recognition by molecularly imprinted xero-gels

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• 作者：Alejandro Simó ; n de Dios ^{simonalejandro@yahoo.es}Author Vitae ; Rosana Badí ; a-Laí ; ñ ; o ^{rbadia@uniovi.es}Author Vitae ; Marta Elena Dí ; az-Garcí ; a ; ^{medg@uniovi.es}Author Vitae
• 关键词：Cancer biomarker ; Neurotransmitters ; Molecularly imprinted xero-gels ; Dummy imprinted xero-gel
• 刊名：Sensors and Actuators B: Chemical
• 出版年：2013
• 出版时间：31 July, 2013
• 年：2013
• 卷：184
• 期：Complete
• 页码：48-53
• 全文大小：735 K

文摘

In this paper, a library of molecularly imprinted xero-gels has been synthesized against the cancer biomarker 5-hydroxyindole acetic acid (5-HIAA) and against the neurotransmitter serotonin (SER) as the target molecules, using different alkoxy-silane precursors. The xero-gel composition, in particular its hydrophobic/hydrophilic balance, is of major importance for adequate recognition and determination of 5-HIAA and SER. The recognition ability of the imprinted xero-gel library is studied using a dynamic flow injection analysis system (FIA system) and the response of each imprinted xero-gel is compared with that for blank materials prepared in the absence of templates. The approach is simple, no-time consuming (no clean-up steps to remove proteins or related molecules), highly sensitive (a detection limit of 5.5 ¡Á 10^?7 M 5-HIAA was found by the standard addition method), reproducible and the calibration graph (y = 5.0 ¡Á 10⁶ ¡Á (M) + 4.98, r² = 0.999) is linear up to 2 ¡Á 10^?4 M 5-HIAA. Results demonstrate that a ¡°dummy¡± imprinted xero-gel against serotonin is highly selective for recognition of 5-HIAA. The use of this ¡°dummy¡± material as recognition phase in an optosensing device is described, while practical application of the system is demonstrated by determining 5-HIAA in synthetic urine samples.