Antinociceptive activity and mechanism of action of hydroalcoholic extract and dichloromethane fraction of Amphilophium crucigerum seeds in mice

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• 作者：Samira Dal Toé ; De Prá ; ^a ; ^{samiradaltoe5@gmail.com} ; Paula Ronsani Ferro^a ; ^{ronsaniferropaula@gmail.com} ; Alessandra Marcon Milioli^a ; ^{alessandramilioli@hotmail.com} ; Flá ; via Karine Rigo^a ; ^{flakrigo@hotmail.com} ; Orlando Justo Chipindo^a ; ^{scotlandia1@hotmail.com} ; Camila Camponogara^b ; ^{miladp@zipmail.com.br} ; Rosana Casoti^c ; ^d ; ^{rocasoti@gmail.com} ; Melâ ; nia Palermo Manfron^d ; ^{melpm10@yahoo.com.br} ; Sara Marchesan de Oliveira^b ; ^{saramarchesan@hotmail.com} ; Juliano Ferreira^e ; ^{ferreija99@gmail.com} ; Gabriela Trevisan^a ; ^f ; ^{gabitrev@hotmail.com}
• 关键词：ALT ; alanine aminotransferase ; ANOVA ; analysis of variance ; AST ; aspartate aminotransferase ; BSA ; bovine serum albumin ; Ca2+) ; calcium ; CFA ; complete Freund's adjuvant ; Crd ; crude extract ; Dcm ; dichloromethane fraction ; DMSO ; dimethyl sulfoxide ; ED50 ; effective dose 50% ; HTAB ; hexadecyltrimethylammonium bromide ; i.g. ; intragastric IC50 ; inhibitory concentration 50% ; Imax ; maximal inhibition ; MPO ; myeloperoxidase ; NAGase ; N-acetyl-β-D-glucosaminidase ; PBS ; phosphate buffer solution ; PWT ; paw with
• 刊名：Journal of Ethnopharmacology
• 出版年：2017
• 出版时间：4 January 2017
• 年：2017
• 卷：195
• 期：Complete
• 页码：283-297
• 全文大小：1978 K
• 卷排序：195

文摘

The medicinal plant generally known as monkey's comb (Amphilophium crucigerum) has been popularly described for the treatment of neuropathic and inflammatory pain, specially seeds preparations.Aim of the studyThe goal of the present study was to evaluate the antinociceptive effect of the crude extract (Crd) and dichloromethane fraction (Dcm) of A. crucigerum seeds, and investigate the involvement of transient receptor potential vanilloid 1 (TRPV1) receptor in this effect.Materials and methodsMale Swiss mice were used in this study. The effects of Crd and Dcm was tested on capsaicin-induced Ca2+ influx or the specific binding of [3H]-resiniferatoxin. Moreover, after treatment with Crd or Dcm, animals were exposed to acute pain (hot water tail-flick and capsaicin intraplantar test) or chronic pain models (injection of complete Freund's adjuvant or partial ligation of the sciatic nerve). Acute adverse effects were also noted: locomotor activity, corporal temperature, hepatic or renal damage, gastrointestinal transit alteration, and ulcerogenic activity.ResultsThe oral administration of Crd or Dcm resulted in an antinociceptive effect in the hot water tail-flick (48 °C) and capsaicin intraplantar tests. Furthermore, these preparations exhibited antinociceptive and anti-inflammatory effects in a chronic inflammatory pain model, and antinociceptive effects in a neuropathic pain model. Moreover, Crd and Dcm reduced capsaicin-induced Ca2+ influx and diminished the [3H]-resiniferatoxin specific binding to spinal cord membranes. Acute adverse events were not found with Crd or Dcm administration.ConclusionIn conclusion, our results support the analgesic effect of A. crucigerum and suggest the presence of compounds that may act as TRPV1 antagonists.