Pituitary adenylate cyclase-activating peptide (PACAP) and PAC₁ receptor in the testis of cartilaginous fish Torpedo marmorata: A molecular and phylogenetic study

详细信息    查看全文

• 作者：Marisa Agnese ; Salvatore Valiante ; Luigi Rosati ; Piero Andreuccetti ; ^{piero.andreuccetti@unina.it" class="auth_mail" title="E-mail the corresponding author} ; Marina Prisco
• 关键词：PACAP ; PAC1R ; Torpedo marmorata ; Spermatogenesis ; Steroidogenesis
• 刊名：Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：191
• 期：Complete
• 页码：26-35
• 全文大小：2681 K

文摘

The role of PACAP in spermatogenesis and steroidogenesis has been largely investigated in last years in mammals; conversely, a few studies have been performed in non mammalian vertebrates. In this paper we investigated the sequence, expression and localization of PACAP and its PAC₁ receptor in the testis of the benthic elasmobranch Torpedo marmorata, the marbled electric ray. Cloning a partial PACAP cDNA, we demonstrated for the first time in elasmobranches that PACAP shows a highly conserved sequence, compared with the PACAP of other chordates (tunicates and vertebrates). Moreover, the phylogenetic analysis revealed that PACAP has been well preserved during evolution and that a negative selection acts on PACAP sequence, leading to the conservation of the coding sites. The phylogenetic consensus tree showed also that Torpedo PACAP is more related with the amphibian PACAP than with the teleost one.

Finally, we demonstrated that in T. marmorata PACAP and its PAC₁ receptor are synthesized directly in the testis, where they show a wider localization than mammals, suggesting that this neuropeptide is involved in the control of Torpedo spermatogenesis.