Polyphasic taxonomic characterization of Lactobacillus rossiae

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• 作者：Scheirlinck Ilse ; Van der Meulen Roel ; Vrancken Gino ; De Vuyst Luc ; Settanni Luca ; V ; amme Peter ; Huys Geert
• 关键词：Lactobacillus rossiae ; Phes ; Rpoa ; (GTG)₅-PCR ; Polyphasic taxonomy
• 刊名：Systematic and Applied Microbiology
• 出版年：2009
• 出版时间：April 2009
• 年：2009
• 卷：32
• 期：2
• 页码：151-156
• 全文大小：236 K

文摘

(GTG)₅-PCR fingerprinting and pheS sequence analysis of 18 Lactobacillus rossiae isolates, mainly originating from Belgian and Italian artisan sourdoughs, revealed intraspecies grouping as evidenced by the delineation of three and two subgroups, respectively. On the other hand, 16S rRNA and rpoA gene sequence analysis and DNA–DNA hybridizations supported the accommodation of all isolates in a single species. No correlation between genetic and phenotypic heterogeneity was observed. Collectively, these data do not warrant taxonomic division of L. rossiae. On the other hand, the considerable differences in intraspecies sequence variation of L. rossiae isolates displayed by the pheS (9.8 % ) and rpoA (1.1 % ) genes highlight that the discriminatory power of housekeeping genes as alternative genomic markers for the 16S rRNA gene in the identification of Lactobacillus species may significantly differ from gene to gene. In conclusion, this study has demonstrated that a polyphasic approach remains highly useful for identification of isolates belonging to genotypically heterogeneous species such as L. rossiae.