Differential DJ-1

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• 作者：Ravindran Kumaran ; Jana V ; rovcova ; Connie Luk ; Simone Sharma ; Alan Renton ; Nicholas W. Wood ; John A. Hardy ; Andrew J. Lees ; Rina B ; opadhyay
• 关键词：DJ-1 mRNA ; Parkinson's disease ; Quantitative real-time PCR ; Post-mortem human brain
• 刊名：Neurobiology of Disease
• 出版年：2009
• 出版时间：November 2009
• 年：2009
• 卷：36
• 期：2
• 页码：393-400
• 全文大小：531 K

文摘

Mutations in the DJ-1 gene have been linked with rare cases of early onset, autosomal recessive Parkinson's disease (PD). To determine whether DJ-1 is also involved in the pathogenesis of common forms of PD we have compared DJ-1 mRNA levels in a number of post-mortem PD and control brain regions using quantitative real-time PCR. Region-specific decreases were observed in DJ-1 mRNA levels in putamen, frontal cortex, parietal cortex and cerebellum in PD ( 30–60 % ) compared to controls whilst an up-regulation was observed in the amygdala ( 90 % ) and entorhinal cortex ( 39 % ). Using quantitative western blot analysis, parallel decreases in DJ-1 protein levels were seen in frontal cortex, putamen and cerebellum of PD cases. By using 2-dimensional gel electrophoresis, we show preponderance of acidic pI isoforms of DJ-1 monomer in PD vulnerable regions, namely frontal cortex and medulla suggestive of differential post-translational modifications. Our findings point to a putative role of DJ-1 in the pathogenesis of PD.