Characterization of coproporphyrinogen III oxidase in Plasmodium falciparum cytosol

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• 作者：Viswanathan Arun Nagaraj ; Dasari Prasad ; Rajavel Arumugam ; Pundi N. Rangarajan ; Govindarajan Padmanaban
• 关键词：Coproporphyrinogen III oxidase ; Plasmodium falciparum ; Heme
• 刊名：Parasitology International
• 出版年：2010
• 出版时间：June 2010
• 年：2010
• 卷：59
• 期：2
• 页码：121-127
• 全文大小：1140 K

文摘

A unique hybrid pathway has been proposed for de novo heme biosynthesis in Plasmodium falciparum involving three different compartments of the parasite, namely mitochondrion, apicoplast and cytosol. While parasite mitochondrion and apicoplast have been shown to harbor key enzymes of the pathway, there has been no experimental evidence for the involvement of parasite cytosol in heme biosynthesis. In this study, a recombinant P. falciparum coproporphyrinogen III oxidase (rPfCPO) was produced in E. coli and confirmed to be active under aerobic conditions. rPfCPO behaved as a monomer of 61 kDa molecular mass in gel filtration analysis. Immunofluorescence studies using antibodies to rPfCPO suggested that the enzyme was present in the parasite cytosol. These results were confirmed by detection of enzyme activity only in the parasite soluble fraction. Western blot analysis with anti-rPfCPO antibodies also revealed a 58 kDa protein only in this fraction and not in the membrane fraction. The cytosolic presence of PfCPO provides evidence for a hybrid heme-biosynthetic pathway in the malarial parasite.