Multi-disciplinary methods to define RNA-protein interactions and regulatory networks

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• 作者：Manuel Ascano¹ ; Stefanie Gerstberger¹ ; Thomas Tuschl ^{ttuschl@rockefeller.edu ttuschl@mail.rockefeller.edu}
• 刊名：Current Opinion in Genetics & Development
• 出版年：2013
• 出版时间：February, 2013
• 年：2013
• 卷：23
• 期：1
• 页码：20-28
• 全文大小：2103 K

文摘

The advent of high-throughput technologies including deep-sequencing and protein mass spectrometry is facilitating the acquisition of large and precise data sets toward the definition of post-transcriptional regulatory networks. While early studies that investigated specific RNA-protein interactions in isolation laid the foundation for our understanding of the existence of molecular machines to assemble and process RNAs, there is a more recent appreciation of the importance of individual RNA-protein interactions that contribute to post-transcriptional gene regulation. The multitude of RNA-binding proteins (RBPs) and their many RNA targets has only been captured experimentally in recent times. In this review, we will examine current multidisciplinary approaches toward elucidating RNA-protein networks and their regulation.