Separation and identification of phenolic acids from some species of the Asteraceae family using HPLC with using diode array detector
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文摘
The inportance of pharmaceuticals of natural orgin is increasing recently; their sources are mainly medicinal plants. The search is directed on raw materials rich in pharmacologically active compounds, especially those easily isolated. To these belong the plants from the species Chrysanthemum (Fam. Asteraceae) which have been systematically investigated. The objective of the present study is the isolation and identification of phenolic acids and their quantitative determination. The plant material (100g) was extracted with chloroform in a Soxhlet apparatus to remove chlorophyll and other ballast compounds. The purified material was dried at room temperature and then extracted with methanol until colourless extracts were obtained and then with 1:1 mixture of methanol and water. The extracts were evaporated to a constant volume (100ml). Before analysis the methanolic extracts were purified by solid phase extraction (Octadecyl, 500mg, J.T. Baker USA). The analysis was curried out using HPLC and diode array detector (DAD). The separation conditions were optimalized using a commercial “Drylab G” software (LC Research Lafayette USA). The separated components were identified on the basis of their retention times, UV spectra and purity parameters (PuP) of standards. The qualitative composition of the extracts (Chrysanthemum maximum Ram. (DC), Chrysanthemum segetum L., Rudbeckia laciniata L.) was established by comparison of the UV spectra with those of the standards. The quantitative analysis of methanolic extracts of the species Chrysanthemum segetum L. was carried out for the available standards using the method of external standard (ES) and the multicomponent analysis (MCA) available in Varian's “Polyview” program. The results obtained with the ES and MCA methods were compared.