Effects of exogenous enzymes on in vitro gas production kinetics and ruminal fermentation of four fibrous feeds

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• 作者：M.M.Y. Elghandour^a ; A.Z.M. Salem^a ; ^d ; ^{asalem70@yahoo.com} ; M. Gonzalez-Ronquillo^a ; J.L. Bó ; rquez^a ; H.M. Gado^b ; N.E. Odongo^c ; C.G. Peñ ; uelas^a
• 关键词：ADFom ; acid detergent fibre ; CP ; crude protein ; DM ; dry matter ; DMD ; DM degradability ; ENZ ; the ZADO® ; enzyme preparation mixture ; GP ; gas production ; GY24 ; gas yield at 24  ; h of incubation ; OM ; organic matter ; OMD ; in vitro OM digestibility ; MCP
• 刊名：Animal Feed Science and Technology
• 出版年：2013
• 出版时间：31 January, 2013
• 年：2013
• 卷：179
• 期：1-4
• 页码：46-53
• 全文大小：294 K

文摘

This study was conducted to investigate effects of increasing doses: 0 (control), 6 (low), 12 (medium) and 24 (high) mg/g DM of ZADO^? enzyme preparation mixture (ENZ) on in vitro gas production (GP) and some ruminal fermentation parameters of the fibrous feeds Saccharum officinarum (leaves), Andropogon gayanus (leaves), Pennisetum purpureum (leaves) and Sorghum vulgare (straw). Rumen liquor was obtained from two Brown Swiss cows fitted with permanent rumen cannulae fed a total mixed ration of a 500:500 commercial concentrate and alfalfa hay ad libitum. The GP was recorded at 2, 4, 6, 8, 10, 12, 24, 48, 72 and 96 h of incubation. After 96 h, the incubation was stopped and the pH of the mixture was determined and filtrate used to determine dry matter degradability (DMD), partitioning factor (PF₉₆), gas yield (GY₂₄), in vitro organic matter digestibility (OMD), metabolizable energy (ME), short chain fatty acids (SCFA), and microbial crude protien production (MCP). In general, the crude protein (CP) content of the fibrous feeds was low and ranged from 23 g/kg DM (S. officinarum) to 44 (A. gayanus). The fibre contents (i.e., NDFom and ADFom) were highest (P<0.05) in S. officinarum. Increasing ENZ dose linearly increased (P<0.05) GP of all fibrous feeds and had a quadratically increased (P<0.05) asymptotic gas production in P. purpureum and S. vulgare and rate of gas production in S. officinarum and S. vulgare. Addition of ENZ also quadratically increased (P<0.05) GP at all incubation times in S. officinarum and S. vulgare, and A. gayanus, but only at 72 h in A. gayanus. The parameters of ruminal fermentation of OMD, ME, GY₂₄ and SCFA linearly increased (P<0.05) and MCP linearly decreased (P<0.05) with the ENZ addition. Addition of enzyme affected ruminal fermentation of our feeds differently, mainly dependent on their fibre content, although dosage of enzyme was also important as impacts generally increased at higher dosages of ENZ.