Oxidized low-density lipoprotein induces the production of superoxide by neutrophils
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- 作者：Maeba ; Ryouta ; Maruyama ; Akiko ; Tarutani ; Osamu ; Ueta ; Nobuo ; Shimasaki ; Hiroyuki
- 关键词：Neutrophil ; Oxidized LDL ; Superoxide ; Chemiluminescence ; Lipid peroxidation ; Atherosclerosis
- 刊名：FEBS Letters
- 出版年：1995
- 出版时间：December 27, 1995
- 年：1995
- 卷：377
- 期：3
- 页码：309-312
- 全文大小：409 K
文摘
Exposure of guinea pig peritoneal neutrophils to ox-LDL led to the production of superoxide, which was measured by the formation of superoxide-dependent chemiluminescence. The cells exposed to unoxidized LDL, e.g. native LDL, acetyl-LDL, and self-aggregates of LDL showed no production of superoxide. The superoxide production was correlated with the levels of oxidative modification of LDL and reached a maximum between 10 and 30 min during incubation, but preincubating the cells with cytochalasin B decreased the superoxide production. These findings indicate that neutrophils rapidly take up ox-LDL by phagocytosis and generate superoxide which may cause superoxide-mediated lipid peroxidation in vivo.