Bioprocess for the production of recombinant HAP phytase of the thermophilic mold Sporotrichum thermophile and its structural and biochemical characteristics

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• 作者：Anay Kumar Maurya ; Deepak Parashar ; T. Satyanarayana ; ^{tsnarayana@gmail.com}
• 关键词：Sporotrichum thermophile ; P. pastoris ; Recombinant phytase ; Circular dichroism ; Site-directed mutagenesis ; Fluorescence quenching
• 刊名：International Journal of Biological Macromolecules
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：94
• 期：part_PA
• 页码：36-44
• 全文大小：2388 K
• 卷排序：94

文摘

A ∼41-fold improvement in rStPhy production has been achieved under constitutive GAP promoter as compared to that of wild strain S. thermophile. Analysis of surface properties of rStPhy revealed the critical factors that determine thermostability of the protein. The catalytically important amino acids (Arg74, His75, Arg78, His368 and Asp369) were identified by docking and site directed mutagenesis studies.