Development of multiplex assay with 16 SNPs on X chromosome for degraded samples

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• 作者：Takahito Oki ; Tokutaro Hayashi ; Masao Ota ; Hideki Asamura ; ^{asamura@shinshu-u.ac.jp}
• 关键词：X chromosomal SNPs ; Multiplex single base extension reactions ; Degraded DNA ; Shorter PCR products
• 刊名：Legal Medicine
• 出版年：2012
• 出版时间：January 2012
• 年：2012
• 卷：14
• 期：1
• 页码：11-16
• 全文大小：624 K

文摘

We selected 16 new X chromosomal SNPs (rs4827155, rs471205, rs7884160, rs16982419, rs985251, rs3813932, rs6630351, rs4132871, rs5966270, rs7471388, rs6641116, rs6521038, rs5990560, rs5959408, rs414960, and rs3006142) and developed the two X chromosomal SNPs Octaplex systems using multiplex single base extension reactions. To make the systems more useful for analyzing degraded DNA samples, we designed primers to render amplicons of 100 bp or shorter (shorter PCR products). Statistical analyses of the 16 SNPs indicated a high usefulness for the Japanese forensic practice. In addition, results of tests on degraded DNA confirm the usefulness of this technique in such samples.