Hotspot mutations in polyomavirus positive and negative Merkel cell carcinomas

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• 作者：Tuukka Veija^a ; Virinder Kaur Sarhadi^a ; Virve Koljonen^b ; Tom Bohling^a ; Sakari Knuutila^a ; ^{sakari.knuutila@helsinki.fi" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Merkel cell carcinoma ; next-generation sequencing ; mutations
• 刊名：Cancer Genetics
• 出版年：2016
• 出版时间：January-February 2016
• 年：2016
• 卷：209
• 期：1-2
• 页码：30-35
• 全文大小：257 K

文摘

Merkel cell polyomavirus (MCV) infection underlies most Merkel cell carcinoma (MCC), a primary neuroendocrine carcinoma of the skin. While previous research has focused on MCV-positive MCC tumors, less is known about the oncogenesis in MCV-negative tumors.

In this study, we analyzed mutational status of 27 MCC tumors with known MCV status for hotspot regions of 50 cancer-related genes by targeted next-generation sequencing using the Ion AmpliSeq Cancer Hotspot Panel.

In addition to previously reported TP53, KIT, and PIK3CA gene mutations, we found somatic mutations in the tyrosine kinase domain of the EGFR gene in a small proportion of the cells in six tumor tissues. RB1 mutations were seen only in virus negative tumors.

Hotspot mutations were more frequent in MCV-negative tumors, although the difference was not statistically significant. No clear hotspot mutation profile was observed. Novel RB1 mutations were detected only in MCV-negative tumors.